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ListView.SeparatorVisibility = SeparatorVisibility.None; is being ignored on both iOS and Android ever since we have upgraded to Xamarin.Forms 22.214.171.124. The separator lines are visible on both platforms, and there basically is no difference between SeparatorVisibility.Default and SeparatorVisibility.None anymore.
After some more research, it turns out that ListViewCachingStrategy.RecycleElement is actually the cause of this issue, not necessarily the update to 126.96.36.199.
With ListViewCachingStrategy.RetainElement there is no separator (with ListView.SeparatorVisibility = SeparatorVisibility.None), but with ListViewCachingStrategy.RecycleElement there suddenly is.
*** This bug has been marked as a duplicate of bug 39802 ***